Cloned (Comment) | Organism |
---|---|
expressed in Escherichia coli AD494 and BL21(DE3) cells | Escherichia coli |
Protein Variants | Comment | Organism |
---|---|---|
G127A | mutation has no obvious effect on enzymatic activity | Escherichia coli |
K214R | the mutant exhibits an about 25% decrease in enzyme activity compared to the wild type enzyme | Escherichia coli |
K214R/K281R | the mutant exhibits an about 25% decrease in enzyme activity compared to the wild type enzyme | Escherichia coli |
K281R | the mutant exhibits an about 12% decrease in enzyme activity compared to the wild type enzyme | Escherichia coli |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Escherichia coli | - |
- |
- |
Purification (Comment) | Organism |
---|---|
Ni-NTA column chromatography | Escherichia coli |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
acetyl-CoA + N-hydroxy-2-aminofluorene | - |
Escherichia coli | CoA + N-acetyloxy-2-aminofluorene | - |
? | |
acetyl-CoA + N-hydroxy-2-aminofluorene | - |
Escherichia coli AD494 | CoA + N-acetyloxy-2-aminofluorene | - |
? | |
additional information | the enzyme is deacetylated by CobB | Escherichia coli | ? | - |
? | |
additional information | the enzyme is deacetylated by CobB | Escherichia coli AD494 | ? | - |
? |
Synonyms | Comment | Organism |
---|---|---|
N-hydroxyarylamine O-acetyltransferase | - |
Escherichia coli |
NhoA | - |
Escherichia coli |